Viscosity and Wettability of Hyaluronic Acid according to Antimicrobial Supplementation, Ionic Strength, and pH
고홍섭, 장지연, 김윤영, 박문수,
소속 상세정보
고홍섭 ( Kho Hong-Seop ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
장지연 ( Chang Ji-Youn ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
김윤영 ( Kim Yoon-Young ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
박문수 ( Park Moon-Soo ) - Gangneung-Wonju National University College of Dentistry Department of Oral Medicine and Diagnosis
KMID : 0355220140390030090
Abstract
Purpose: To investigate viscosity and wettability of hyaluronic acid (HA) solutions according to supplementation of lysozyme and/or peroxidase, and different ionic strength and pH conditions.
Methods: Solutions containing HA were prepared using distilled deionized water (DDW) and simulated salivary buffer (SSB) in different conditions. Different concentrations of hen eggwhite lysozyme and bovine lactoperoxidase was added into HA solutions. HA solutions with antimicrobials in different ionic strength and pH conditions were prepared. Viscosity was measured using cone-and-plate digital viscometer at six different shear rates and wettability on acrylic resin and Co-Cr alloy was determined by contact angle.
Results: The viscosity values of HA dissolved in DDW were decreased in order of HA, HA containing lysozyme, HA containing peroxidase, and HA containing lysozyme and peroxidase. The viscosity values for HA in DDW were decreased as the concentration of lysozyme and/or peroxidase increased. However, the viscosity values for HA in SSB showed no significant changes according to the concentration of lysozyme and/or peroxidase. The viscosity values of HA solutions were inversely proportional to ionic strength and pH. The contact angle of HA solutions showed no significant differences according to tested surface materials, addition of lysozyme and/or peroxidase, and different ionic strength and pH conditions. Contact angles on acrylic resin by HA solutions in all tested conditions were much higher than those by human saliva.
Conclusions: The rheological properties of HA supplemented with lysozyme and/or peroxidase in different ionic strength and pH conditions were objectively confirmed, indicating the possibility of HA with lysozyme and/or peroxidase as main components in the development of effective saliva substitutes.
키워드
Hyaluronic acid; Lysozyme; Peroxidase; Saliva substitute; Viscosity; Wettability
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